Otolaryngology Imaging Core  

Zeiss LSM 5 Pascal - Axiovert 200M

Zeiss LSM 5 Pascal - Axiovert 200M
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Like all LSM units of the fifth generation, the hall- mark of LSM 5 PASCAL is a fully motorized scanning module. All moving components, such as emission filter wheels, main and secondary dichroic beam splitters, the pinhole and the mechanical attenuators for each laser line, are computer-controlled and take up the required position making the set-up error free and without any need for cumbersome manual operation. A special benefit in practice: the position can be reset automatically at the press of a button.

Inverted Microscope:

Filter Sets

 

Important features

DAPI  (#31013)  (standard)

Excitation: 360-370 nm, dichroic: 380 nm LP, emission: 435-485 nm.

FITC  (#41001)  (standard)

Excitation: 460-500 nm, dichroic: 505 nm LP, emission: 510-560 nm.

TRITC (#41002C)  (standard)

Excitation: 530-560 nm, dichroic: 570 nm LP, emission: 590-650 nm.

Cy5 (#41023)  (standard)

Excitation: 630-675 nm, dichroic: 680 nm LP, emission: 685-735 nm.

mCherry (#49008) (swappable)

Excitation: 540-580 nm, dichroic: 585 nm LP, emission: 595-665 nm.

GFP (#49002)  (swappable)

Excitation: 450-490 nm, dichroic: 495 nm LP, emission: 500-550 nm.

Objectives

ZEISS Objective Assistant »

Lasers

Type

Wavelength nm

Power

Argon

458, 488, 514

25mW

HeNe 543

543

1mW

HeNe633

633

5mW

Software

Brochures

  1. Pascal Brochure
  2. Pascal Userguide
  3. AxioVision
  4. AxioCam HR

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